Relationship of Physical Factors to the Freezing Damage to Red Cells during Freezing and Thawing.
Abstract
Methylene blue uptake by red cells of ACD blood decreases linearly between the first and ninth week of storage at 1 C. The rate of decrease is not affected by addition of adenine and inosine, but is apparently accelerated by weekly mixing. Methylene blue uptake is significantly related to the posttransfusion survival of red cells (p < 0.01). These changes are considered to be a function of length of storage. The content of G-6-PDH stored for ten weeks at 1 C shows no significant changes and it is not affected by addition of adenine and inosine added during storage, or by weekly mixing. The phospholipid content of red cells of all frozen and thawed specimens of blood is significantly lower than the values obtained in fresh blood, the mean loss being 42.3% with a range of 21.7 to 53.1%. Significantly higher values of phospholipids are obtained with addition of 40 mg. of glycerol per 100 ml of the 15% lactose and 10% dextrose solution. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 15, 1970
- Accession Number
- AD0864784
Entities
People
- Max M. Strumia