Preservation of Insect Cells in Liquid Nitrogen

Abstract

Aedes aegypti cells grown as suspension cultures and Aedes albopictus cells grown as monolayer cultures have been frozen and stored in liquid nitrogen with very little loss of viability. Cultures frozen in medium containing 8% dimethylsulfoxide (DMSO) yielded 93% viable A. aegypti cells after storage in liquid nitrogen for 1 month. A. albopictus cells preserved similarly yielded a full cell sheet within 4 to 6 days after thawing and were successfully carried through three transfers with no apparent loss of viability.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1970
Accession Number
AD0866110

Entities

People

  • Bruce L. Brown
  • Charles D. Rapp
  • James D. Lehman
  • Stanley C. Nagle Jr.

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Alanine
  • Amino Acids
  • Anatomy
  • Animal Structures
  • Anti-Bacterial Agents
  • Aspartic Acid
  • Biological Pigments
  • Biological Sciences
  • Cell Line
  • Cells
  • Culture Techniques
  • Maryland
  • Monomolecular Films
  • Neutral Amino Acids
  • Nitrogen
  • United States
  • Viability

Readers

  • Oncology (Cancer Research).
  • Trauma Surgery or Emergency Medicine.
  • Vector-Borne Disease and Entomology