Requirements for Cholesterol, Hematin, and Lecithin for Optimal Growth of a Porcine Kidney Cell Line

Abstract

The nutritional requirements for growth of a porcine kidney (PK) cell line in a chemically defined medium were studied in cells grown as monolayer cultures in T-30 Falcon plastic flasks incubated at 36 C with caps tightened. The PK cell appeared to be unique among a variety of heteroploid cell lines in its requirement for a number of unusual substances. Successful propagation of PK cells was obtained in a serum-free defined medium that contained cholesterol, hematin lecithin, and coenzyme. The PK cell line may serve as a useful tool in a study of intermediary lipid metabolism at the cellular level.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1970
Accession Number
AD0866111

Entities

People

  • Kiyoshi Higuchi

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Albumins
  • Amino Acids
  • Anatomy
  • Biological Sciences
  • Cell Line
  • Cells
  • Cells (Biology)
  • Chemistry
  • Cholesterol
  • Coenzymes
  • Culture Techniques
  • Cultured Cells
  • Encephalitis
  • Lipid Metabolism
  • Lipids
  • Metabolism
  • Monomolecular Films

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Molecular and Cellular Biochemistry
  • Oncology (Cancer Research).