Relationship of Oxidation-Reduction Potential to Growth of Tissue Culture Media Poised Before Incubation

Abstract

Earlier observations revealed that incubation of media and the attendant changes in oxidation-reduction potential (ORP) were related to improved cell production. This is a report of work done to show that the higher levels and increased rates of growth of cells grown in incubated medium are associated with the ORP level of the medium before its inoculation with cells. Work was done using 250-ml centrifuge spinner bottles as the culture vessels. Further work is needed to establish the desirability of deliberate poising of media prior to use for studies in small vessels and flasks.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1970
Accession Number
AD0866115

Entities

People

  • John F. Rosensteel
  • Luis H. Garcia
  • William F. Daniels

Tags

Communities of Interest

  • Ground and Sea Platforms

DTIC Thesaurus Topics

  • Amino Acids
  • Cell Count
  • Centrifuges
  • Confidence Limits
  • Culture Media
  • Electrodes
  • Essential Amino Acids
  • Incubation
  • Inoculation
  • Instrument Panels
  • Maryland
  • Observation
  • Oxidation
  • Production
  • Rubber Adhesives
  • Stainless Steel
  • Tissue Culture

Readers

  • Aquatic Ecology
  • Systems Analysis and Design
  • Virology (or Medical Virology).