Growth of L Cells in a Chemically Defined Medium in a Controlled Environment Culture System,
Abstract
Equipment has been developed to permit monitoring and automated control of environmental variables such as pH, temperature, pO2, pCO2, and redox potential in order to study their effects on the growth and metabolism of cultured mammalian cells. A battery of six water-jacketed 500-ml Bellco spinner flasks was instrumented to provide (by electrode probes) information on pH, pO2, and redox potential of each culture during growth. Stepping switches and motorized valves coupled to the sensing probes permitted control of the environment. Studies with automated control of pO2 levels in L cell cultures showed that dissolved O2 tensions of about 9% were optimal for cell growth. At pO2 values of 5 and 20%, peak cell yields as well as growth rates were reduced by approximately 20%. Peak yields of L cell cultures exceeded 5,000,000 cells per ml when grown for 4 days without medium renewal from inocula of 1 plus or minus 500,000 cells per ml in a defined medium sparged with 5% CO2 and adequate O2 to maintain 9% dissolved O2 tension. The redox potentials of L cell cultures reflected the pO2 levels in the medium and ranged from -25 to +150 mv (calomel reference) for O2 values ranging from 2 to 20% dissolved oxygen tension.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1970
- Accession Number
- AD0866576
Entities
People
- Gordon W. Taylor
- John P. Kondig
- Kiyoshi Higuchi
- Stanley C.