Relationship between Plaque Assay and Mouse Assay for Titrating Rift Valley Fever Virus

Abstract

A plaque assay method for the small-plaque variant of the pantropic van Wyk strain of Rift Valley fever virus is described. The ease in preparation, the reproducibility of results, the distinctness of the plaques, and the relatively short time required for plaques to appear make the method highly suitable for use in the laboratory. A model was constructed utilizing this technique for estimating MICLD/50 values from plaque-forming units. The model obtained has provided a firm basis for a large reduction in costs of routine assays, due to its relative costs of mouse and tissue culture assay.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1970
Accession Number
AD0868150

Entities

People

  • Bill G. Mahlandt
  • Frederick Klein
  • Ralph E. Lincoln
  • Sarah L. Eyler

Tags

DTIC Thesaurus Topics

  • Arboviruses
  • Assays
  • Cells
  • Culture Techniques
  • Laboratory Animals
  • Linearity
  • Maryland
  • Materials
  • Michigan
  • Monomolecular Films
  • Reproducibility
  • Rift Valley Fever
  • Rift Valleys
  • Tissue Culture
  • United States
  • Valleys
  • Viruses

Fields of Study

  • Biology

Readers

  • Aerospace Test and Evaluation
  • Virology (or Medical Virology).