Concentration of Rift Valley Fever and Chikungunya Viruses by Precipitation with Ammonium Sulfate and Potassium Aluminum Sulfate

Abstract

A simple and efficient method for concentrating Rift Valley fever and chikungunya viruses is described. Ammonium sulfate and potassium aluminum sulfate are used as precipitating agents, and the precipitate is resuspended to volumes suitable for further processing and purification. Concentration of both viruses by one or the other sulfate may be obtained this way. The method permits concentration of live Rift Valley fever and chikungunya viruses about 100-fold with negligible losses of virus. Rift Valley fever virus retained a higher level of infectivity with potassium aluminum sulfate, but chikungunya virus retained a higher infectivity level with ammonium sulfate. The data indicate that serum plays an important role in the concentration of both viruses.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1970
Accession Number
AD0868151

Entities

People

  • Bill G. Mahlandt
  • Frederick Klein
  • Ralph E. Lincoln
  • Ralph R. Cockey

Tags

Communities of Interest

  • C4I

DTIC Thesaurus Topics

  • Adsorption
  • Animal Diseases
  • Blood Proteins
  • Chemistry
  • Influenza
  • Laboratory Animals
  • Literature
  • Materials
  • Potassium
  • Precipitates
  • Precipitation
  • Rift Valley Fever
  • Rift Valleys
  • United States
  • Valleys
  • Virion
  • Viruses

Readers

  • Electrochemical Engineering/ Fuel Cell Technologies
  • Immunology
  • Infectious Disease/Epidemiology