Purification and Physical Properties of Eastern Equine Encephalitis Virus
Abstract
A new purification procedure was adopted for eastern equine encephalitis virus that does not subject the virus to pelleting at any stage. Three- to four-liter volumes are passed through a DEAE cellulose column. The virus-containing fractions are banded on a sucrose cushion and finally banded isopycnally in a linear sucrose gradient. This method reduces the volume 1000- fold with a concomitant increase in viral titer. Numerous criteria were used to establish that this viral preparation is essentially free from cellular debris and nonviral material. Physical studies on this purified viral product were initiated. The sedimentation coefficient by the band sedimentation technique was 240 S. The calculated buoyant density in sucrose was 1.18 g/cc. The diameter of the virus was 54 millimicron. From the diameter and the buoyant density it is possible to calculate the molecular weight of a spherical particle: for eastern equine encephalitis virus, it is 58 x 1,000,000 daltons.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1970
- Accession Number
- AD0868354
Entities
People
- Anthony A. Fuscaldo
- Edwin J. Hoffman
- Halvor G. Aaslestad