Separation of Donor and Recipient Bacteria by Column Chromatography
Abstract
When donor and recipient strains of Escherichia coli were added to columns containing Cellex-P (a cation-exchange cellulose), more than 80% of the female cells passed through the column but only 11% or less of the male cells were eluted. However, when donor strains were blended prior to their addition to the column, the majority of these cells were eluted. These results indicated that the filamentous appendages termed F pili (which are removed by blending) were responsible for the adherence of donor cells to the cellulose.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1970
- Accession Number
- AD0868355
Entities
People
- George E. Fulk
- Robert M. Zsigray
- William D. Lawton