Chromosome Mapping of Pasteurella Pseudotuberculosis by Interrupted Mating
Abstract
Pasteurella pseudotuberculosis containing the plasmid F'lac transferred its chromosome from a specific origin in an oriented manner to five multiply auxotrophic strains of P. pseudotuberculosis. In a mating system containing gelatin, glucose, and phosphate buffer, a maximum of 0.1% of the donor cells transferred lead markers. The donor population was counterselected with nalidixic acid. P. pseudotuberculosis containing the plasmids F'CM, F'13, or F'his donated markers in the same order and with approximately the same entry times as did the standard F'lac donor strain, suggesting that F itself has enough homology with a specific region on the chromosome of P pseudotuberculosis to mobilize the chromosome. We have established the entry time (in minutes after mixing parental cultures) of seven markers as follows: proline (10 minutes); arginine (14 minutes); histidine (14 minutes); threonine (25 minutes); lysine (50 minutes); tyrosine (67 minutes); and tryptophan (77 minutes). The resulting chromosome map shows a marked similarity both in relative order and in distance between markers to the well established chromosome map of Escherichia coli.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1970
- Accession Number
- AD0869310
Entities
People
- Harold B. Stull
- William D. Lawton