An Improved Heat-Stable Glutamine-Free Chemically Defined Medium for Growth of Mammalian Cells
Abstract
A heat-stable chemically defined medium, free of glutamine, is described for the growth of mammalian cells in suspension culture. The presence of L-alanine in the defined medium permitted the omission of glutamine. A 22- fold increase in the population of a substrain of mouse L cells was obtained in 6 days with no medium replenishment during incubation. Maximum yields were obtained by daily medium replacement and venting of cultures. Growth was also improved in a line of cat kidney cells and HeLa cells, and in another substrain of L cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1970
- Accession Number
- AD0872692
Entities
People
- Bruce L. Brown
- Stanley C. Nagle Jr.