Preliminary Studies on the Growth of Chikungunya Virus Using Baby Hamster Kidney Cell Suspension Cultures
Abstract
This report describes the methods used to obtain high titers of chikungunya virus using suspension cultures of BHK-21 (clone 13) cells. The cells were grown at 37 deg C to a cell concentration of 1,000,000 to 2,000,000/ml. Following maximum cell growth, the cells were inoculated with chikungunya virus at a multiplicity of 1 to 2 SMICLD50 per cell in the spent Eagle's minimum essential medium for suspension cultures (MEMS), or the cell cultures were centrifuged at 1,000 rpm and resuspended in either fresh MEMS or medium 199 prior to inoculation. The medium used had no effect on virus titer. The inoculated cultures were incubated at 34 deg C until the cell viability dropped to 30%, which usually occurred 28 to 30 hours post-inoculation. Following these procedures, chikungunya virus titers of 10.3 to 11.8 log10 SMICLD50/ml were obtained.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1970
- Accession Number
- AD0873140
Entities
People
- Howard M. Hodge
- Jack L. Davis
- William E. Campbell Jr.