Measuring Equilibrium Binding Affinity of Biological Macromolecules in Solution by Thermophoresis
Abstract
The primary research focus of the San Diego State University (SDSU) Structural Biochemistry Laboratory led by Dr. Tom Huxford is tounderstand how the assembly of macromolecular complexes gives rise to fundamental healing processes such as inflammation in response totrauma or infection and the repair of damaged muscles. The goal of this proposal was to secure funds for the acquisition and implementationof a NanoTemper Technologies Monolith NT.115 Thermophoresis instrument. The instrument, which is one of the first of its kind to beacquired and implemented within the state of California, permits rapid and accurate measurement of equilibrium binding affinities ofbiological macromolecules in solution. This qauntitative information plays a vital role in supporting the static structural data that researchersin the lab typically obtain through x-ray crystallography. Thus far, the instrument has been used to measure dimerization, DNA binding, andco-activator binding by transcription factor NF-kappaB binding. Initial studies have also begun that are attempting to measure theinteraction of myosin motor proteins with the muscle assembly and repair chaperone protein UNC-45.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 18, 2015
- Accession Number
- AD1001109
Entities
People
- Tom Huxford
Organizations
- San Diego State University