The Parkinson Transcriptome Project: Roadmap to Personalized Care
Abstract
This study provides a comprehensive catalog of gene expression - both protein-coding and non-coding - specifically in dopamine neurons in situ in human brain. Going beyond traditional messenger RNA sequencing, all polyadenylated and nonpolyadenylated transcripts over 50 bases were deeply sequenced using ribo-depleted RNA from dopamine neurons laser captured from human post-mortem brains of patients with Parkinson's disease using laser-capture RNA sequencing. We show unequivocally that non-coding RNAs are abundantly transcribed in human dopamine neurons and highlight significant changes in the expression of both protein-coding and non-coding transcripts in patients with clinical and neuropathological Parkinson's disease. Expression Quantitative Trait Locus analysis indicates that the expression of many non-coding and protein-coding transcripts in nigral dopamine neurons is associated with genetic variants. Finally, these pilot phase data support the feasibility of measuring select RNAs linked to Parkinson's in vulnerable dopamine neurons as possible biomarkers in blood.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2015
- Accession Number
- AD1001193
Entities
People
- Clemens R. Scherzer
Organizations
- Brigham and Women's Hospital