Annexin A2 in Proliferative Vitreoretinopathy
Abstract
Proliferative vitreoretinopathy (PVR) is a potentially blinding disease that occurs in almost one-half of military personnel who suffer a penetrating wound to the eye. When there is a tear in the retina, cells inside the eye (retinal pigmented epithelial [RPE] cells) begin to proliferate and, over time, can form a scar that pulls the remaining retina away from the back of the eye, compromising vision. We are examining the potential role of the calcium-regulated, phospholipid-binding protein, annexin A2(A2) in PVR. In a robust model of PVR, we find that mice lacking A2 have a greatly diminished scarring response to intraocularinjury. At early time points, we see a lack of migration of RPE cells into the vitreous space in the A2-deficient mouse, and, later time points, the degree of scarring and retinal detachment appear to greatly diminished in these animals. We have developed a method for the isolation of mouse RPE cells, and are studying their directed migration in vitro in response to macrophage-specific signals. In addition, we have approvals in place and have set up procedures for examining expression of A2 and related molecules in PVR membranes from human patients with PVR due to retinal surgery. Going forward, we plan to expand on these studies by identifying the macrophage-dependent molecular cues that direct RPE migration, and define the specific role played by A2 in this process.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2015
- Accession Number
- AD1001744
Entities
People
- Katherine A Hajjar
Organizations
- Weill Cornell Medicine