Spider Silk: From Protein-Rich Gland Fluids to Diverse Biopolymer Fibers
Abstract
The primary objective of this research is to elucidate the interactions, mechanisms and biochemistry of the spider silk producing process at the molecular level. Our primary focus is to characterize the protein-rich fluid in the various spider silk producing glands. We have been using a battery of magnetic resonance methods including solution and solid-state nuclear magnetic resonance (NMR) and micro imaging (MRI) in combination with wide angle and small angle X-ray diffraction (WAXD and SAXD) techniques at Argonne National Laboratory (ANL) to probe silk protein structure and dynamics prior to and following fiber formation. We have established a number of methods for isotopically (2H/13C/15N) enriching the silk proteins during the course of our AFOSR funding that have allowed us to investigate the structure, dynamics, and organization of spider silk protein within the silk gland and in the final spun fiber with a range of magnetic resonance methods. We successfully developed magnetic resonance imaging (MRI) techniques with localized spectroscopy to probe the silk glands of spiders and map protein structure through out the silk gland.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 06, 2016
- Accession Number
- AD1001844
Entities
People
- Gregory P. Holland
- Jeffery L Yarger
Organizations
- Arizona State University