Improving Joint Function Using Photochemical Hydrogels for Articular Surface Repair

Abstract

The goal of our research is to introduce a novel means to regenerate the articular cartilage and restore normal function of the joint. A strategy that can generate durable hyaline articular cartilage, which will be predominantly type II collagen and, is capable of integrating with the surrounding cartilage matrix (without fissures) could improve the long-term outcome of joint surface repair. Several photochemically crosslinkable gels have been developed and tested in vitro and in vivo in miceincluding collagen, fibrin, PEG thiol-ene, and gelatin methacrylamide. All have shown satisfactory cell viability and some cartilage formation. A modification to using dynamic culture as a bioreactor has resulted in the formation of aggregates of chondrocytes called dynamic Sel-Regenrating cartialge (dSRC) that can be encapsulated in the gels. This has resulted in superior cartilage formation and has been tested in two swine in a pilot study. Long-term swine studies are underway.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2015
Accession Number
AD1002222

Entities

People

  • Mark A. Randolph
  • Thomas J. Gill

Organizations

  • Massachusetts General Hospital

Tags

DTIC Thesaurus Topics

  • Bioengineering
  • Cartilage
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Confocal Microscopy
  • Culture Media
  • Culture Techniques
  • Engineering
  • Health Services
  • Mechanical Properties
  • Pilot Studies
  • Polymers
  • Stem Cells
  • Three Dimensional

Readers

  • Immunology and Pathology
  • Polymer Science and Technology
  • Trauma Surgery or Emergency Medicine.

Technology Areas

  • Biotechnology