Novel Therapy for Bone Regeneration in Large Segmental Defects
Abstract
The purpose of this study is to test the efficacy of thrombopoietin (TPO) to heal a segmental bone defect (SBD) in a large animal model, the minipig. The scope of the research comprises the following specific aims (i) to determine the union rate of tibial midshaft defects in minipigs treated with BMP-2, TPO, or saline control; and (ii) to evaluate the safety and side effects of treating tibial midshaft defects in minipigs treated with BMP-2, TPO, or saline control. In the previous research period we completed surgeries and collection of tissue/datafrom 27 minipigs which had surgeries. We completed a pilot study to work out the surgical and equipment logistics and completed 12 minipig surgeries (plus the 4 pilot minipig surgeries). As we had to wait until all minipigs were euthanized before completing the majority of the analyses, we have only just begun these preparations. We have confirmed the quality of our blood samples and will begin blood chemistry analysis this reporting period. That said, the most significant findings during this period is that TPO is able to heal the critical size defect in minipigs. Our xray data show significant improvement compared to saline treated controls. We however also determined that our scaffolds were not weight bearing and with a different fixation method we believe we can even further improve TPOs efficacy which will be another goal in Year 3.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2015
- Accession Number
- AD1002366
Entities
People
- Jeffrey Anglen
- Melissa A. Kacena
- Tien-min Chu