Proteomic Analysis of Trauma Induced Heterotopic Ossification Formation
Abstract
Heterotopic Ossification (HO), the ectopic formation of bone in soft tissues, has been found to complicate >60% of extremity war injuries in casualties from Afghanistan and Iraq. Elevated levels of circulating and local cytokines released in response to high energy blast injuries have been found to correlate with the onset of HO, indicating that the disease process will require early intervention; however, preventive therapies such as tissue radiation, bone morphogenetic protein antagonists, and cyclooxygenase inhibitors, carry substantial risks for patients recovering from orthopaedic trauma. Consequently, there is an as yet unmet medical need to develop assays of serum and wound fluid biomarkers to identify those patients at greatest risk of HO progression during their recovery. The current project is using liquid chromatography/mass spectroscopy, in combination with other cell and protein biological assays, to evaluate the serum and wound fluid from civilian and military orthopaedic trauma patients for the presence of cytokines or factors capable of inducing HO. The studies are focusing on a select set of candidate biochemical pathways (bone morphogenetic, cyclic AMP, Wnt) that have been implicated in genetic models of HO. The research team includes expertise in civilian and military orthopaedic surgery,adipose and bone marrow stromal/stem cell biology, proteomics and mass spectroscopy, and regenerative medicine. During the first year of the project, the scope of the study has expanded to include the analysis of serum samples from established murine (burn) and rat (blast injury) models of HO and these analyses will be used to complement and support the initially proposed studies of human serum.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2015
- Accession Number
- AD1002377
Entities
People
- Jeffrey M. Gimble
Organizations
- Tulane University of Louisiana