Separation of VX, RVX and GB Enantiomers Using Liquid ChromatographyTime-of-Flight Mass Spectrometry
Abstract
Chemical nerve agents such as VX (O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothioate), RVX (S-[2-(diethylamino)ethyl] O-isobutyl-methylphosphonothioate), and GB (isopropyl methylphosphonofluoridate) exist as a mixture of two enantiomers. A Lux 5u Cellulose-1 normal-phase chiral liquid chromatography column (Phenomenex; Torrance, CA) was used to separate the enantiomers for all V and G agents within 15 min. Atmospheric pressure chemical ionization mode was used for liquid chromatographytime-of-flight mass spectrometry analysis. For large-scale separation and quantitation, the UV absorbance at 210 nm (with a bandwidth of 4 nm) was referenced to a wavelength of 360 nm. An Agilent series 1200 fraction collector (Agilent Technologies; Santa Clara, CA) was used to collect both (+) and () enantiomers for each chemical agent separated. Identification and isolation of each enantiomer of a chemical agent is very beneficial for in vitro and in vivo toxicological studies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2016
- Accession Number
- AD1002738
Entities
People
- Mark D Winemiller
- Sue Y. Bae
Organizations
- Edgewood Chemical Biological Center