Impact of Noncoding Satellite Repeats on Pancreatic Cancer Metastasis
Abstract
The goal of the project is to understand the role of HSATII satellite repeat expression in pancreatic cancer metastatic potential. An inducible over-expression vector was created and was successfully used in cancer cell lines with evidence of transcriptional changes and increased migratory capability consistent with a role in metastasis. HSATII was also assessed in pancreatic circulating tumor cells (CTCs), which are enriched for metastatic precursors. Initial results find these cells in patients with preneoplastic IPMN lesions suggesting a blood based early detection biomarker. Unexpectedly, a novel reverse transcriptional machinery has been identified with HSATII expression and this results in genomic expansion of these pericentromeric repeats in cancer. This has provided a new understanding of HSATII regulation and function, which has adjusted the goals of the project to address these new findings.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2015
- Accession Number
- AD1003169
Entities
People
- Daniel Haber
- David T Ting
Organizations
- Massachusetts General Hospital