Identifying microRNAs that Regulate Neuroblastoma Cell Differentiation
Abstract
In this reporting period, we mainly focused on characterizing another novel differentiation-inducing miRNA, miR-449a. We examined the differentiation-inducing function of miR-449a in multiple neuroblastoma cell lines. We have demonstrated that miR-449a functions as an inducer of cell differentiation in neuroblastoma cell lines with distinct genetic backgrounds, including the MYCN amplification status. We further show that miR-449s mimic induces cell cycle exit, induces cell apoptosis, and decreases the capacity of the neuroblastoma cells to form colonies. Our results support the strong tumor suppressive function of miR-449a in neuroblastoma cells, providing the experimental evidence for developing miR-499a-based differentiation therapy for neuroblastoma.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2015
- Accession Number
- AD1003301
Entities
People
- Alexander Pertsemlidis
- Liqin Du
- Xiuye Ma
- Zhenze Zhao
Organizations
- University of Texas at San Antonio