Hyperpolarized 13C MR Markers of Renal Tumor Aggressiveness
Abstract
RCCs have a wide range of aggressiveness, which is currently difficult to assess noninvasively. There is an unmet clinical need to reliably distinguish RCC from benign renal tumors for optimal triage of therapies. Hyperpolarized (HP) 13C magnetic resonance (MR) is a new metabolic imaging approach that is capable of interrogating specific enzymatic pathways in real time. Our aim was to interrogate tumor metabolism in more clinically relevant RCC tumor models by employing patient-derived tumor tissues in conjunction with HP MR. We have shown that in addition to increased lactate production, the clear cell subtype of RCC (which makes for the majority of RCCs) has a distinct feature of increased lactate efflux which can be used to discern it from benign renal tumors in the ex vivo bioreactor set-up. Additionally we have shown that while patient derived renal tissue slices used in orthotopic mouse model can be established successfully, it is not feasible to conduct hyperpolarized MR experiments due to the limited tumor growth of only the high grade aggressive tumor tissues. Nonetheless in the process, we have used human immortalized RCC cells in the orthotopic mouse model and developed pulse sequences to interrogate increased lactate production as well as efflux using HP13C MR.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2015
- Accession Number
- AD1004078
Entities
People
- Renuka Sriram
Organizations
- University of California, San Francisco