A Novel Tumor Antigen and Foxp3 Dual Targeting Tumor Cell Vaccine Enhances the Immunotherapy in a Murine Model of Renal Cell Carcinoma

Abstract

A major barrier in vaccine therapy is represented by the presence of immunosuppressive factors predominant in cancer patients, such as regulatory T cells (Tregs) and suppressive myeloid cells, including myeloid derived suppressor cells(MDSCs) and tumor associated macrophages (TAMs). Here we report a tumor cell vaccine designed to target both tumor cells and Tregs by using Foxp3, a Treg-functional protein as an antigen in tumor cell vaccine. During first year of the project, we have demonstrated that the dual target vaccine had anti-tumor activity against established tumor. During last year, we tested a combination strategy to target suppressive myeloid cells (MDSCs and TAMs) with a pharmacological approach, tasquinimod, combined with dual targeting vaccine. The combination strategy prolonged survival, compared to vaccine single treatment. Overall, our results suggest that targeting immunosuppressive cells with vaccine or pharmacological strategies, results in greater anti-tumor activity and therapeutic efficacy, and provides foundation to test the strategy in clinical setting for patients with advanced, metastatic kidney cancer.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2015
Accession Number
AD1004091

Entities

People

  • Shen Li

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Blood
  • Blood Cells
  • Bone Marrow Cells
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Culture Techniques
  • Diseases And Disorders
  • Immune System
  • Kidney Cancer
  • Lymphatic System
  • Lymphocytes
  • Macrophages
  • Medical Personnel
  • Mononuclear Phagocyte System
  • Myeloid Cells

Fields of Study

  • Biology
  • Medicine

Readers

  • Molecular and Cellular Biology
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech