The Role of the Rab Coupling Protein in ErbB2-Driven Mammary Tumorigenesis and Metastasis
Abstract
During the Year-2 we focused in our understanding of the role of the Rab Coupling Protein (RCP) in ErbB2-mediated lung metastasis formation. To accomplish this, we have derived both proficient and deficient ErbB2 stable expressed cell lines and then performed orthotopic mammary fat pad and tail vein cell injection using the classic immunodeficient NCr athymic mouse model as syngenic recipient. Consistent with our previous in vivo data, the overexpression of RCP led into drastically decrease the number of lung. In contrast, the depletion of RCP using RNA interference approach significantly increase the number of lung metastases.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2015
- Accession Number
- AD1004095
Entities
People
- Cynthia Lavoie
- Douglas Strathdee
- Gordon B. Mills
- Jason Turpin
- Jim C. Norman
- Julie M. Livingstone
- Julie-emilie Huot-marchand
- Laura Jones
- Lewis A Chodosh
- Louise Mitchell
- Michael Hallett
- Morag Park
- Pierre-luc Boulay
- Shirley Campbell
- Shreya Mitra
- Virginie Sanguin-gendreau
- William Muller
Organizations
- McGill University