Developing Novel Therapeutics Targeting Undifferentiated and Castration-Resistant Prostate Cancer Stem Cells
Abstract
The main objective of this DOD-supported project is to identify and develop novel therapeutics to target the undifferentiated (PSA-/lo), castration-resistant PCSCs. We proposed to achieve this objective with two Specific Aims: 1) To perform phage display library (PDL) screening in PSA-/lo PCa cells to identify PCSC-specific homing peptides; and 2) To perform unbiased drug library screening to identify novel PCSC-targeting chemicals. In the past year, we have made good progress in accomplishing the goals for both Aims. For Specific Aim 1, we have further characterized the JRM2 peptide. For Specific Aim 2, we have finished a targeted library screening to identify several compounds that could sensitize the AR-PSA- LNCaP cells. We have also started screening our UNIQUE PSA-/lo CRPC cells against a Protein Kinase Inhibitor Collection that contains >750 compounds that target >140 kinases. In the second year, we shall continue several experiments in both Aims, especially testing the in vitro and in vivo cytotoxicities of the conjugated JRM2 peptide and finishing up screening and validating the kinase inhibitor library screening.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2015
- Accession Number
- AD1004100
Entities
People
- Dean Tang
Organizations
- The University of Texas MD Anderson Cancer Center