Identification of the Gene for Scleroderma in the Tsk/2 Mouse Strain: Implications for Human Scleroderma Pathogenesis and Subset Distinctions
Abstract
This project is focused on an animal model of the human disease, systemic sclerosis (SSc), called Tsk2/+. The SSc-like traits in Tsk2/+ heterozygotes are highly penetrant. With their readily apparent skin fibrosis resulting from ECM anomalies, Tsk2/+ mice have signs that resemble human SSc features, making it useful as a pre-clinical model. In this report, we show a clear time dependence on the gene expression in the skin of the Tsk2/+ mice. We have pinpointed a mutation in Col3a1 that is the Tsk2 gene, and have confirmed the sequence difference between Tsk2/+ and the parent strain, 101/H. We present results on the expression of TGFbeta mRNA from cells cultured from Tsk2/+ and WT littermates that suggest a mechanism for the up-regulation of TGFbeta seen in the mutant strain. We show that elastin content in the skin, known to be controlled by TGFbeta and possibly up-regulated in SSc, is the earliest indicator of tight-skin in the tissue. Finally, we show that Tsk2/+ mice, and mouse fibroblasts transfected with Col3a1 from Tsk2/+, share a substantial fibrotic gene expression program compared to WT mice or transfectants, indicating that expression of the Col3a1(Tsk2) gene alone accounts for the trait in Tsk2/+ mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2015
- Accession Number
- AD1004917
Entities
People
- Carol Artlett
- Elizabeth P. Blankenhorn
- Michael L. Whitfield
Organizations
- Dartmouth College