Inhibition of Orthopaedic Implant Infections by Immunomodulatory Effects of Host Defense Peptides

Abstract

We first developed a murine model of orthopaedic implant infection that will lead to future studies utilizing transgenic and knock out mice to understand the mechanisms that contribute to the effects of bacteria on implant osseointegration. Both S. aureus and A. baumanii were found to impair implant osseointegration by inducing local osteolysis. This reconciles confusion in the literature regarding the effects of A. baumanii. The effects of A. baumaniion osseointegration were found to require quorum sensing. This will lead to future studies to determine whether quorum sensing antagonists that are being developed by other investigators will block the effects of A. baumanii on osseointegration. It was also found that the IDR peptide reduces the effects of S. aureus on implant osseointegration. This will lead to future studies to optimize the use of IDR peptides in our murine model of implant infection; in larger, more clinically relevant, animal models; and ultimately in human trials.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2014
Accession Number
AD1004927

Entities

People

  • Edward M. Greenfield

Organizations

  • Case Western Reserve University

Tags

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Bacteria
  • Bacterial Infections
  • Biological Sciences
  • Biomedical Research
  • Bone Diseases
  • Chemical Synthesis
  • Chemistry
  • Culture Media
  • Data Sets
  • Gram-Negative Bacterial Infections
  • Infection
  • Medical Personnel
  • Microbiology
  • Staphylococcus Aureus
  • Statistical Analysis
  • Wound Infections

Readers

  • Infectious Disease/Epidemiology
  • Neurotrauma and Rehabilitation Medicine.
  • Oncology (Cancer Research).