Rescue of TET2 Haploinsufficiency in Myelodysplastic Syndrome Patients Using Turbo Cosubstrate

Abstract

We continued with our focus on the large scale expression of TET2 dioxygenase. During this period we developed a convenient assay for TET2 dioxygenase, which will be utilized to determine the kinetics of wild-type and various clinical mutants of TET2 dioxygenase. Further, we made a library of putative 2-oxoglutarate analogs which will be used to rescue the activity of TET2 mutants from MDS patient. Initial results shows that the activity of TET2 can be modified using 2-oxoglutarate analogs.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2015
Accession Number
AD1007201

Entities

People

  • Mridul Mukherji

Organizations

  • University of Missouri System

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Amino Acids
  • Biomedical Research
  • Cell Line
  • Cells
  • Culture Techniques
  • Department Of Defense
  • Electronic Mail
  • Hematologic Diseases
  • Kinetics
  • Liquid Chromatography
  • Mass Spectrometers
  • Mass Spectrometry
  • Neoplasms
  • Proteins
  • Spectrometry
  • Standards
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  • Analytical Chemistry
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