In Vitro Endocrine Disruption Screening of 3-nitro-1,2,4-triazol-5-one (NTO)

Abstract

The compound, 5-nitro-1,2,4-triazol-3-one, (NTO) is an explosive that performs similar to RDX but has reduced sensitivity compared to RDX. Routine safety assessments including toxicity testing have been performed with NTO. NTO has been demonstrated to affect testes weights in orally dosed rats. One possible mode of action for adverse testicular effects is hormonal (endocrine) disregulation. A series of in vitro bioassays were performed to assess if NTO disrupts the endocrinesystem. The assays performed were estrogen receptor binding, androgen receptor binding, estrogen transactivation, aromatase, and steroidogenesis. The endpoints of these assays contribute to a weight of evidence (WoE) strategy for determining NTO endocrine disruption. No NTO effects on the tested in vitro endpoints were observed and using a WoE approach, NTO does not appear to directly affect testosterone or estrogen mediated endocrine regulation.

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Document Details

Document Type
Technical Report
Publication Date
Sep 25, 2012
Accession Number
AD1007533

Entities

People

  • Valerie H. Adams

Organizations

  • United States Army Public Health Command

Tags

DTIC Thesaurus Topics

  • Alkenes
  • Androgen Receptors
  • Androgens
  • Anti-Bacterial Agents
  • Bioassay
  • Biochemistry
  • Cell Line
  • Cells
  • Chemistry
  • Contracts
  • Health
  • Hormones
  • Microsomes
  • Plastic Bonded Explosives
  • Public Health
  • Standards
  • Testes

Readers

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