Depth-Resolved Nanospray Desorption Electrospray Ionization Mass Spectrometry in Biofilms
Abstract
The goal of this project was to develop a nano-DESI sensor which can be used to obtain in situ, depth-resolved analyses of metabolites and possibly proteins from biofilms at different depth. A microcapillary system was developed and tested for this goal. The microcapillary system delivered a solvent (ethanol) from one capillary while the other capillary collected and transported a charged liquid to the mass spectrometer inlet. We tried different configurations of microcapillary systems and optimized follow rates and its operation. The project successfully demonstrated that a dual microcapillary system can be used to collect nanoliter sample from the biofilms at different depth. However, the microcapillary system also collected cells which failed its use for MS.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 16, 2015
- Accession Number
- AD1008200
Entities
People
- Erhan Atci
- Haluk Beyenal
- Tim Ewing
Organizations
- Washington State University