Development of a Microfluidic Platform to Analyze Evolution of Programmed Bacterial Death

Abstract

Evolution of programmed cell death in bacteria is a poorly understood phenomenon in biology. A critical limitation is the lack of high through put technology to examine dynamics of small bacterial populations. To address this limitation, we proposed to develop a drop let based microfluidic technology to generate population bottleneck. This platform will serve as a critical foundation for our long-term goal to develop a quantitative understanding of PAD evolution by using engineered bacteria. For the proposed pilot study, we will focus on the technology development. In particular, our proposed work consists of following specific tasks, focusing on proof-of-concept demonstration of this device and its use for analyzing engineered bacteria.

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Document Details

Document Type
Technical Report
Publication Date
Dec 20, 2015
Accession Number
AD1008634

Entities

People

  • Lingchong You

Organizations

  • Duke University

Tags

Communities of Interest

  • Energy and Power Technologies

DTIC Thesaurus Topics

  • Abstracts
  • Agreements
  • Bacteria
  • Biomaterials
  • Cell Physiological Processes
  • Cells
  • Computational Biology
  • Department Of Defense
  • Dynamics
  • Engineering
  • Mathematics
  • Molecules
  • Personal Information Managers
  • Pilot Studies
  • Programmed Cell Death
  • Students
  • Systems Biology

Fields of Study

  • Biology
  • Environmental science

Readers

  • Military/Explosive Ordnance Disposal (EOD) Technology
  • Oncology (Cancer Research).
  • Theoretical Analysis.