Development of Real-Time Reverse Transcriptase PCR Assays for the Detection of Punta Toro Virus and Pichinde Virus

Abstract

Research with high biocontainment pathogens such as Rift Valley fever virus (RVFV) and Lassa virus (LASV) is expensive, potentially hazardous, and limited to select institutions. Surrogate pathogens such as Punta Toro virus (PTV) for RVFV infection and Pichinde virus (PICV) for LASV infection allow research to be performed under more permissive BSL-2 conditions. Although used as infection models, PTV and PICV have no standard real-time RT-PCR assays to detect and quantify pathogenesis. PTV is also a human pathogen, making a standardized detection assay essential for biosurveillance. Here, we developed and characterized two real-time RT-PCR assays for PICV and PTV by optimizing assay conditions and measuring the limit of detection (LOD) and performance in multiple clinical matrices.

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Document Details

Document Type
Technical Report
Publication Date
Sep 09, 2016
Accession Number
AD1009281

Entities

People

  • Christopher P. Stefan
  • David A. Kulesh
  • Jeffrey W. Koehler
  • Kitty Chase
  • Susan Coyne
  • Timothy D Minogue

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

DTIC Thesaurus Topics

  • Acids
  • Biomedical Research
  • Culture Techniques
  • Detection
  • Diseases And Disorders
  • Infection
  • Infectious Diseases
  • Nucleic Acids
  • Polymerase Chain Reaction
  • Proteins
  • Public Health
  • Rift Valley Fever
  • Rift Valleys
  • Rodents
  • Standards
  • United States
  • Viruses

Fields of Study

  • Biology

Readers

  • Infectious Disease/Epidemiology
  • Systems Analysis and Design
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology