Intracellular Detection of Viral Transcription and Replication Using RNA FISH

Abstract

Many hemorrhagic fever viruses require BSL-3 or 4 laboratory containment for study. The necessary safety precautions associated with this work often contribute to longer assay times and lengthy decontamination procedures. In this Chapter, we will discuss recent advances in RNA fluorescence in situ hybridization (FISH) that not only allow entirely new investigations into the replication of these viruses, but also how this method can be applied to any virus with a known sequence and how it can be rapidly performed to minimize time spent in high containment. We have adapted existing protocols for mRNA detection with appropriate changes for examining viruses in a variety of containment laboratories.

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Document Details

Document Type
Technical Report
Publication Date
May 26, 2016
Accession Number
AD1009531

Entities

People

  • Michael E. Lindquist

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

DTIC Thesaurus Topics

  • Albumins
  • Alcohols
  • Aluminum Foil
  • Amides
  • Biological Staining And Labeling
  • Culture Techniques
  • Detection
  • Hybridization
  • Infection
  • Lassa Fever
  • Materials
  • Methanols
  • Microscopy
  • Molecules
  • Viruses
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Systems Analysis and Design
  • Virology (or Medical Virology).