Method for Processing Liver Spheroids Using an Automatic Tissue Processor

Abstract

In support of the Ex vivo Console of Human Organoids (ECHO) program, the personnel at the Molecular Toxicology Branch of the U.S. Army Edgewood Chemical Biological Center developed a specialized method for paraffin-embedding liver organoids. Processing the organoids in this manner enables subsequent microtome sectioning to observe the internal structures. Because the tissues that are usually processed by an automatic tissue processor are far larger than organoids, which are typically only 1 mm in diameter, an alternate method from that described in the instrument manual was required. The key step that enabled processing of organoids was to first increase their size by embedding them in a plug of agarose gel. This tissue-processing method, which was developed for the preparation of organoids, is described in this report.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2016
Accession Number
AD1009565

Entities

People

  • Harry Salem
  • Janna S. Madren-whalley
  • Leah R. Valdes
  • Robert L. Kristovich
  • Russell M. Dorsey

Organizations

  • Edgewood Chemical Biological Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Alkanes
  • Anatomy
  • Automatic
  • Cassettes
  • Cells
  • Centrifuges
  • Department Of Homeland Security
  • Diameters
  • Education
  • Embedding
  • Histology
  • Homeland Security
  • Laboratory Equipment
  • Medical Personnel
  • Microscopy
  • Tissues
  • Toxicology

Readers

  • Computer Science.
  • Critical Infrastructure Protection in CBRN and WMD Threats.
  • Medical Imaging.