Characterization of Two Cysteine Transfer RNA Genes from Xenopus Laevis
Abstract
A DNA fraction enriched in X. laevis tRNA genes was separated from its genomic DNA by CsCl density gradient centrlfugation in the presence of actlnomycln D. The presence of tRNA genes in this fraction was detected by Southern blot analysis after digestion with restriction endonuclease Eco RI and hybridization with kinase labeled tRNA. The DNA fragments were separated by size on 1% agarose gel in which DNA fragments 1600-1800 bp long were eluted from the gel and cloned into the plasmid pBR 325. One clone which hybridized to kinase labeled tRNA was identified and shown to produce a tRNA size product when transcribed in vitro using Xenopus S-100 cell extract.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 12, 1984
- Accession Number
- AD1010717
Entities
People
- Shirley S. Lee
Organizations
- Uniformed Services University of the Health Sciences