Partial Complementation of the UV Sensitivity of Deinococcus Radiodurans Excision Repair Mutants by the Cloned denv Gene of Bacteriophage T4
Abstract
Deinococcus radiodurans has two endonucleases that incise UV irradiated DNA, UV endonuclease-alpha and UV endonuclease-beta, that are believed to functionally overlap. Both endonucleases must be mutationally inactivated to yield an incisionless, markedly UV-sensitive phenotype. denV, the bacteriophage T4 gene encoding pyrimidine dimer-DNA glycosylase (PD-glycosylase), was introduced and expressed via duplication insertion in D. radiodurans wild type, and single and double UV endonuclease mutants. Expression of denV increased UV survival of an incisionless double mutant and the strain deficient only in UV endonuclease-beta. The strain deficient in UV endonuclease-a has wild-type UV-resistance, and the expression of PD-glycosylase exerted no survival effect on this strain or wild-type. These results suggest that UV endonuclease-alpha does not recognize one or more types of cyclobutane dimer incised by the PD-glycosylase or UV endonuclease-beta
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 19, 1990
- Accession Number
- AD1010984
Entities
People
- Hanlong Yao
Organizations
- Uniformed Services University of the Health Sciences