Immunoreactive Changes in the Hypoglossal Nucleus after Nerve Injury

Abstract

Choline acetyltransferase (ChAT) and calcitonin gene-related peptide (CGRP) are synthesized in the hypoglossal motoneuron. This study examines changes in ChAT and CGRP immunoreactivity (IR) after three types of hypoglossal nerve injury (resection, transection and crush) at 1, 3, 7, 20 and 50 days postoperative (dpo). Maximal reduction of ChAT immunostaining occurred at 7 dpo, and by 50 dpo had returned to normal for all injuries. CGRP immunostaining increased maximally at 1-3 dpo and at 50 dpo was normal for nerve crush, yet decreased for transection and resection. Cellular levels of ChAT and CGRP respond in diametric fashion, temporally and in regard to type of injury. Correlative data of reinnervation of tongue musculature were supplied by HRP labeling of hypoglossal motoneurons after nerve injury. These data provide a more complete picture of the role that a neurotransmitter-related protein, ChAT, and a putative trophic neuropeptide, CGRP,may play in nerve regeneration.

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Document Details

Document Type
Technical Report
Publication Date
Jul 25, 1991
Accession Number
AD1010988

Entities

People

  • Maria Curtis

Organizations

  • Uniformed Services University of the Health Sciences

Tags

DTIC Thesaurus Topics

  • Brain
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Endoplasmic Reticulum
  • Health Services
  • Motor Neurons
  • Mrna
  • Nervous System
  • Neurology
  • Neurons
  • Neurosciences
  • Organelles
  • Peptides
  • Peripheral Nervous System
  • Rodents
  • Sciatic Nerve

Fields of Study

  • Biology

Readers

  • Neuroscience