Molecular Interactions of the Hantaan Virus Nucleocapsid Protein
Abstract
Approaches to the determination of the molecular interactions of Hantaan virus nucleocapsid protein (N) are proposed. In its role as the nucleocapsid protein, Ninteracts with viral RNA; however, its specificity for single-stranded or double-stranded nucleic acid and/or nucleotide sequence is unknown. The N protein is alsothought to self-associate, based on previous electron microscopy and immunofluorescence assays. The specific nature of these N-N protein interactions is alsouncharacterized. In addition, the extent of interactions of N with HTV glycoproteins or any other HTV proteins remains to be worked out. The interactions with protein and nucleic acid of the nucleocapsid and capsid proteins from various RNA and DNA viruses are discussed. Finally, potential experimental approaches to determine the molecular interactions of HTV N with itself, with other proteins, with glycoproteins and with nucleic acid are described.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 12, 1991
- Accession Number
- AD1011002
Entities
People
- Darrell Iii E. Griffin
Organizations
- Uniformed Services University of the Health Sciences