Archive of Samples for Long-Term Preservation of RNA and Other Nucleic Acids

Abstract

The project's objective was the development of a scalable all ambient temperature biological sample workflow preserving nucleic acids for molecular analysis. This ambient workflow can be developed for large scale blood collection, transport, and long-term archiving without the use of costly and unreliable cold chain management, using commercially available biopreservation products. For the development of the automated workflow we have chosen Biomatrica's commercially validated products RNAgard Blood tubes, RNAstable and DNAstable Plus, an off the shelf liquid handler (Beckman), acommercially available dryer (Genevac Inc) and small 384 well storage tubes with a commercial tube sealing system for compact storage (Thermo). In this research period we have achieved the final Phase II milestone of processing 500 samples in a week.

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Document Details

Document Type
Technical Report
Publication Date
May 15, 2016
Accession Number
AD1011140

Entities

People

  • Gerald Dodson
  • Vasco Liberal

Tags

Communities of Interest

  • Biomedical
  • C4I

DTIC Thesaurus Topics

  • Acids
  • Birds
  • Centrifuges
  • Chemistry
  • Cost Analysis
  • Cost Reductions
  • Costs
  • Energy
  • Instructions
  • Materials
  • Nucleic Acids
  • Phase
  • Specifications
  • Standards
  • Storage Tubes
  • Test Equipment
  • Transport Ships

Readers

  • Analytical Chemistry
  • Computer Science/Computer Engineering/Data Science/Digital Signal Processing.
  • Distributed Systems and Data Platform Development

Technology Areas

  • Biotechnology