Upstream Transcriptional Regulatory Elements of the S. cerevisiae CSG2 Gene
Abstract
The 5' flanking sequence of CSG2, a Saccharomyces cerevisiae gene that is required for tolerance of high extracellular calcium, was analyzed to identify transcriptional control elements necessary for its expression. Varying amounts of deletions were created in the 5' flanking region of CSG2 with nuclease Bal31 treatment of a CSG2 clone. Deletion plasmids were transformed into yeast and assayed for complementation of the calcium-sensitive growth phenotype of csg2 null mutant cells. The extent of the deletions was determined by gel electrophoresis and sequencing. The result of this analysis is that only about 30 nucleotides upstream of the presumed start codon of the CSG2 gene are needed for its expression as assayed by its ability to rescue the calcium-sensitive phenotype of the null mutant strain.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 07, 1992
- Accession Number
- AD1011149
Entities
People
- Lily Wong
Organizations
- Uniformed Services University of the Health Sciences