The Effect of Cholesterol on the Binding and Insertion of Cytochrome b5 into Liposomes of Phosphatidylcholines
Abstract
Cholesterol regulates the physical properties of lipid bilayers. But despite its extensively characterized effects on phospholipid motional freedom . surface areas, and phase transitions, the effect of cholesterol on protein solubility in lipid bilayers has not been established. To examine if cholesterol affects the solvent properties of lipid bilayers with respect to integral membrane proteins. the spontaneous binding and insertion of cytochrome b5 (cyt b5) into phosphatidylcholine liposomes containing cholesterol has been investigated as an archetypical system for lipid-protein interactions
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 30, 1993
- Accession Number
- AD1011298
Entities
People
- Kenneth M. Taylor
Organizations
- Uniformed Services University of the Health Sciences