Defining Tumor Cell and Immune Cell Behavior in Vivo during Pulmonary Metastasis of Breast Cancer
Abstract
Metastasis is mediated by a complex set of factors, few of which are well understood. While a number of studies over the past several decades have linked function of the immune response to various outcomes in pulmonary metastasis it is currently poorly understood as to how immune cells engage with tumor cells to modulate metastatic success. Over the past two years we have defined a novel network using intravital lung imaging and flow cytometry by which early pioneer tumor cells release large numbers of tumor microparticles (cytoplasm containing blebs) into the lung circulation. We have found these microparticles are rapidly cleared from the lung by local immune cells. The outcome of this is loading of discrete immune populations such as Dendritic Cells with tumor antigen. These cells (dendritic cells specifically) subsequently act restrict successful metastasis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2015
- Accession Number
- AD1011373
Entities
People
- Mark Headley
Organizations
- University of California, San Francisco