Platelet-Derived Growth Factor-BB Stimulates Fibronectin Gene Expression in Fibroblasts Isolated from Rat Thoracic Aorta
Abstract
Platelet-Derived Growth Factor (PDGF) and fibronectin are implicated in cell proliferation and matrix expansion. It is possible that PDGF stimulates cell proliferation via induction of fibronectin synthesis from fibroblasts. Fibroblasts isolated from rat thoracic aorta were used in these studies because they produce a large amount of fibronectin. Therefore. the role of POOF-BS in fibronectin gene expression in cultured fibroblasts was investigated. Northern blot analysis demonstrated that PDGF-BB induces fibronectin mRNA in a time-and dose-dependent manner. Elevated fibronectin mRNA levels were detected at 4 hours and peaked at 6 hours (120% increase, P less than 0.001) after PDGF-BB (20 ng/mI) treatment. The effects of actinomycin D and cycloheximide on fibronectin mRNA synthesis in the presence of PDGF-BB were examined. Actinomycin D blocked the fibronectin mRNA increase induced by PDGF-BB. Cycloheximide produced a greater increase in fibronectin mRNA levels. These results suggest transcriptional and translational control by PDGF-BB.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 13, 1994
- Accession Number
- AD1011392
Entities
People
- Jing Zhang
Organizations
- Uniformed Services University of the Health Sciences