Suppressors (scsl-scs7) of CSG2, a Gene Required by Saccharomyces cerevisiae for Growth in Media Containing 10 mMCa(2+), Identify Genes Required for Sphingolipid Biosynthesis
Abstract
The gene, CSG2, is required for growth of S. cerevisiae cells in media containing10 mM Ca2+. It was cloned, sequenced and found to be an integral membrane protein with nine potential membrane spanning domains and a potential Ca2+ - binding site (EF-hand) predicted to be in a cytosolic loop. To further investigate the function of CSG2p, a collection of mutants (Suppressors of Ca2+ Sensitivity) that suppress the csg2 DELTA 1 Ca2+ -sensitive phenotype was selected at 37 degrees C on YPD + 100 mM Cal2+ plates. These suppressors are recessive and they fall into seven complementation groups (scs1-scs7). All members of the scs1 and scs2 groups simultaneously acquire a Ca2+ (> 10 mM) requirement for growth whereas wild-type cells grow with only trace amounts of Ca2+. This Ca2+ requiring phenotype is not rescued by Sr2+, Mg2+, Co2+, Mn2+, or Ba2+. The SCSI gene was cloned by complementation of the scs1 Ca2+ -requiring phenotype.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1994
- Accession Number
- AD1011395
Entities
People
- Chun Zhao
Organizations
- Uniformed Services University of the Health Sciences