The Expression of Fos, Jun and AP-1 DNA Binding Activity in Rat Supraoptic Nucleus Neurons Following Acute Versus Repeated Osmotic Stimulation

Abstract

FOB and Jun are protein products of their respective proto-oncogenes, c-fos and c-jun. Nuclear concentrations of both proteins rapidly, but transiently, rise after thepresentation of a variety of stimuli under in vitro and in vivo conditions. Fos and Jun can form heterodimeric proteins that act as transcription factors to regulate late response genes.Previous work has demonstrated that hypertonic saline injection results in Fos expression in supraoptic nucleus (SON) neurons that mediate body water homeostasis. Since Fos is thought to act mostly in conjunction with Jun, I first developed a double-label fluorescence immunocytochemical procedure that permits simultaneous visualization of neurons expressing Fos and Jun. After hypertonic saline administration, the immunostaining of c-Fos and c-Jun was coexistent in the majority of rnagnocellular neurons of SON.

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Document Details

Document Type
Technical Report
Publication Date
Jun 22, 1995
Accession Number
AD1011468

Entities

People

  • Ke Wang

Organizations

  • Uniformed Services University of the Health Sciences

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Blood
  • Body Fluids
  • Brain
  • Cells
  • Central Nervous System
  • Chemistry
  • Endocrine Glands
  • Nervous System
  • Neurosciences
  • Neurosecretory Systems
  • Peptide Growth Factors
  • Peptides
  • Pituitary And Hypothalamic Hormones And Analogues
  • Proteins
  • Tissue Extracts
  • Transcription Factors

Fields of Study

  • Biology

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