Targeting Neuronal-like Metabolism of Metastatic Tumor Cells as a Novel Therapy for Breast Cancer Brain Metastasis

Abstract

During this report period, we conducted both intravital imaging and whole tissue clearing based imaging to dissect the interaction between tumor cell and its brain metastatic microenvironment. We have successfully expanded GFAP-GFP mouse line (brain astrocyte specific) and performed preliminary testing on the intravital imaging capability. In addition, we have streamlined and optimized tissue-clearing pipeline. We are able to multiplex staining multiple tissue markers in situ, including brain astrocytes, tumor cell, proliferating cell (Edu) and blood vessel. We have conducted 3D reconstruction of brain metastasis landscape. We will conduct detailed quantifications of large 3D imaging dataset we have obtained. Larger scale in vivo experiments will be conducted in the coming year.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2016
Accession Number
AD1011679

Entities

People

  • Siyuan Zhang

Organizations

  • University of Notre Dame

Tags

DTIC Thesaurus Topics

  • Astrocytes
  • Biological Staining And Labeling
  • Biomedical Research
  • Blood
  • Blood Vessels
  • Breast Cancer
  • Cancer
  • Cells
  • Data Analysis
  • Data Sets
  • Department Of Defense
  • Metastasis
  • Neoplasms
  • Students
  • Targeting
  • Three Dimensional
  • Two Dimensional

Fields of Study

  • Biology
  • Physics

Readers

  • Image Processing and Computer Vision.
  • Oncology (Cancer Research).
  • Traumatic Brain Injury (TBI) and Cognitive Aging in the Guam and Border Populations Affected by Alzheimer's Disease and Tau-Associated Dementias.