Intra-Articular Lubricin Gene Therapy for Post-Traumatic Arthritis
Abstract
Mankin scoring of safranin-O stained sections showed that there was significant pathology in both treatment groups. However, changes in the LubGFP group tended to be less severe than in the GFP group. Although there was significant overlap at the low end of the scale, the highest scores (9 or greater) were found in the GFP group, where full-thickness lesions were commonly seen. A t-Test indicated a less than significant p value of 0.08 for the group difference. However, the study, which included 7 animals per group, was slightly underpowered; sample size estimates based on the data indicated that 2-3 more animals per group will be needed to reject the null hypothesis. This confirmed the original estimate that a total of 10 animals per group will provide sufficient power. In any case the results thus far are encouraging and we see no reason to alter the treatment protocol going forward. We also confirmed that transgene expression was sustained for 8 weeks in joints injected with AAV-LubGFP immediately after ACLT.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2015
- Accession Number
- AD1011691
Entities
People
- James A. Martin
Organizations
- University of Iowa