The Role of B7 Ligand Interactions During an In Vivo Mucosal Immune Response
Abstract
The nematode parasite, H polygyrus, has been extensively used as a model to study the type 2 in vivo immune response. This response is characterized by the development of IL-4 producing T cells which mediate the development of other allergy associated effector cell populations. Following oral inoculation with H polygyrus larvae, the host rapidly develops a type 2 immune response characterized by intestinal mastocytosis, blood eosinophilia, elevations in T cell IL-2R expression, in situ CD4+ T cell expansion and IL-4 production, and increases in B cell MHC class II expression, MLN GC formation and serum IgE and IgG1 levels. The costimulatory signal provided to naive T helper (Th) cells through CD28/CTLA-4 interactions is required for in vivo 10 cell effector function associated with cytokine production. In this project, I examined whether the two well-characterized ligands for these molecules, B1-1 and B7-2, differentially influence the development of a type 1 or type 2 immune response. Administration of the combination of anti-B1-l and anti-B7-2 mAbs inhibited H polygyrus-induced increases in serum IgG1 and 19E levels, the expansion of mesenteric lymph node (MLN) germinal centers, in situ CD4+ T cell expansion, elevated blood eosinophils, and increased intestinal mucosal mast cells. Similarly, both Abs blocked MLN and Peyer's Patch's patch cytokine gene expression and elevations in MLN T cell derived IL-4 protein secretion.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 22, 1998
- Accession Number
- AD1011831
Entities
People
- Rebecca Greenwald
Organizations
- Uniformed Services University of the Health Sciences