CaMKK2 Inhibition in Enhancing Bone Fracture Healing

Abstract

Fracture healing is a chief medical concern for active-duty military personnel as well as aging combat veterans, highlighting a highly critical need for novel therapies to treat this condition. A recent serendipitous discovery that genetic ablation of Ca2+/calmodulin (CaM) dependent protein kinase kinase 2 (CaMKK2) has duel effects on anabolic and catabolic pathways of bone remodeling has led to the idea that its pharmacological inhibition could serve as an efficacious therapeutic strategy to promote efficient fracture healing. STO-609 is a selective, cell-permeable pharmacological inhibitor of CaMKK2 that has been successfully tested in vivo in mice. Hence the goal of this proposal is to develop STO-609-mediated inhibition of CaMKK2 as an efficacious therapeutic strategy to promote accelerated fracture healing and recovery. During the first phase of the award period, we performed a pilot study to establish the following: (1) Reliable and reproducible surgical procedures for creating a transverse femoral fracture and fixing it with an intramedullary device. (2) The treatment protocol for following the animals for the appropriate follow-up period. (3) The collection and analysis of the femur for assessing fracture healing.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2014
Accession Number
AD1012164

Entities

People

  • Michael Voor
  • Uma Sankar

Organizations

  • Indiana University Bloomington

Tags

DTIC Thesaurus Topics

  • Ablation
  • Active Duty
  • Biomedical Research
  • Bone Fractures
  • Demographic Cohorts
  • Inhibition
  • Inhibitors
  • Medical Personnel
  • Military Personnel
  • Orthopedic Surgical Procedures
  • Pharmacology
  • Pilot Studies
  • Professional Development
  • Recovery
  • Surgery
  • Transverse

Fields of Study

  • Biology
  • Medicine

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Immunology and Pathology
  • Medical or Health Care Field.

Technology Areas

  • Biotechnology